Dynamin A as a one-component division machinery for synthetic cells – Nature Nanotechnology

Spira, F. et al. Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments. eLife 6, e30867 (2017).

Article  Google Scholar 

Allard, J. F. & Cytrynbaum, E. N. Force generation by a dynamic Z-ring in Escherichia coli cell division. Proc. Natl Acad. Sci. USA 106, 145–150 (2009).

Article  CAS  Google Scholar 

Bisson-Filho, A. W. et al. Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division. Science 355, 739–743 (2017).

Article  CAS  Google Scholar 

Pfitzner, A.-K., Moser von Filseck, J. & Roux, A. Principles of membrane remodeling by dynamic ESCRT-III polymers. Trends Cell Biol. 31, 856–868 (2021).

Article  CAS  Google Scholar 

Caspi, Y. & Dekker, C. Dividing the archaeal way: the ancient Cdv cell-division machinery. Front. Microbiol. 9, 174 (2018).

Article  Google Scholar 

Bassereau, P. et al. The 2018 biomembrane curvature and remodeling roadmap. J. Phys. D: Appl. Phys. 51, 343001 (2018).

Article  Google Scholar 

Hurley, J. H. ESCRTs are everywhere. EMBO J. 34, 2398–2407 (2015).

Article  CAS  Google Scholar 

Sundborger, A. C. & Hinshaw, J. E. Regulating dynamin dynamics during endocytosis. F1000Prime Rep. 6, 85 (2014).

Article  Google Scholar 

Lemus, L. & Goder, V. Membrane trafficking: ESCRTs act here, there, and everywhere. Curr. Biol. 32, R292–R294 (2022).

Article  CAS  Google Scholar 

Bohuszewicz, O., Liu, J. & Low, H. H. Membrane remodelling in bacteria. J. Struct. Biol. 196, 3–14 (2016).

Article  CAS  Google Scholar 

Olivi, L. et al. Towards a synthetic cell cycle. Nat. Commun. 12, 4531 (2021).

Article  CAS  Google Scholar 

Schlimpert, S. et al. Two dynamin-like proteins stabilize FtsZ rings during Streptomyces sporulation. Proc. Natl Acad. Sci. USA 114, E6176–E6183 (2017).

Article  CAS  Google Scholar 

Bramkamp, M. Structure and function of bacterial dynamin-like proteins. Biol. Chem. 393, 1203–1214 (2012).

Article  CAS  Google Scholar 

Guo, L. & Bramkamp, M. Bacterial dynamin-like protein DynA mediates lipid and content mixing. FASEB J. 33, 11746–11757 (2019).

Article  CAS  Google Scholar 

Bürmann, F., Ebert, N., van Baarle, S. & Bramkamp, M. A bacterial dynamin-like protein mediating nucleotide-independent membrane fusion. Mol. Microbiol. 79, 1294–1304 (2011).

Article  Google Scholar 

Sawant, P., Eissenberger, K., Karier, L., Mascher, T. & Bramkamp, M. A dynamin-like protein involved in bacterial cell membrane surveillance under environmental stress. Environ. Microbiol. 18, 2705–2720 (2016).

Article  CAS  Google Scholar 

Guo, L., Sattler, L., Shafqat, S., Graumann, P. L. & Bramkamp, M. A bacterial dynamin-like protein confers a novel phage resistance strategy on the population level in Bacillus subtilis. mBio 13, e0375321 (2022).

Article  Google Scholar 

De Franceschi, N. et al. Synthetic membrane shaper for controlled liposome deformation. ACS Nano. 17, 966–978 (2022).

Article  Google Scholar 

Bhatia, T., Christ, S., Steinkühler, J., Dimova, R. & Lipowsky, R. Simple sugars shape giant vesicles into multispheres with many membrane necks. Soft Matter 16, 1246–1258 (2020).

Article  CAS  Google Scholar 

Antonny, B. et al. Membrane fission by dynamin: what we know and what we need to know. EMBO J. 35, 2270–2284 (2016).

Article  CAS  Google Scholar 

Mattila, J.-P. et al. A hemi-fission intermediate links two mechanistically distinct stages of membrane fission. Nature 524, 109–113 (2015).

Article  CAS  Google Scholar 

Dreher, Y., Jahnke, K., Schröter, M. & Göpfrich, K. Light-triggered cargo loading and division of DNA-containing giant unilamellar lipid vesicles. Nano Lett. 21, 5952–5957 (2021).

Article  CAS  Google Scholar 

Steinkühler, J. et al. Controlled division of cell-sized vesicles by low densities of membrane-bound proteins. Nat. Commun. 11, 905 (2020).

Article  Google Scholar 

Kozlovsky, Y. & Kozlov, M. M. Membrane fission: model for intermediate structures. Biophys. J. 85, 85–96 (2003).

Article  CAS  Google Scholar 

Fabrikant, G. et al. Computational model of membrane fission catalyzed by ESCRT-III. PLoS Comput. Biol. 5, e1000575 (2009).

Article  Google Scholar 

Zhang, G. & Müller, M. Rupturing the hemi-fission intermediate in membrane fission under tension: reaction coordinates, kinetic pathways, and free-energy barriers. J. Chem. Phys. 147, 064906 (2017).

Article  Google Scholar 

Gao, M., Huang, X., Song, B. L. & Yang, H. The biogenesis of lipid droplets: lipids take center stage. Prog. Lipid Res. 75, 100989 (2019).

Article  CAS  Google Scholar 

De Franceschi, N. et al. The ESCRT protein CHMP2B acts as a diffusion barrier on reconstituted membrane necks. J. Cell Sci. 132, jcs217968 (2018).

Article  Google Scholar 

Bertin, A. et al. Human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation. Nat. Commun. 11, 2663 (2020).

Article  CAS  Google Scholar 

Pfitzner, A.-K. et al. An ESCRT-III polymerization sequence drives membrane deformation and fission. Cell 182, 1140–1155.e18 (2020).

Article  CAS  Google Scholar 

Schöneberg, J. et al. ATP-dependent force generation and membrane scission by ESCRT-III and Vps4. Science 362, 1423–1428 (2018).

Article  Google Scholar 

Remec Pavlin, M. & Hurley, J. H. The ESCRTs—converging on mechanism. J. Cell Sci. 133, jcs240333 (2020).

Article  Google Scholar 

Abil, Z. & Danelon, C. Roadmap to building a cell: an evolutionary approach. Front. Bioeng. Biotechnol. 8, 927 (2020).

Article  Google Scholar 

Cada, A. K. et al. Friction-driven membrane scission by the human ESCRT-III proteins CHMP1B and IST1. Proc. Natl Acad. Sci. USA 119, e2204536119 (2022).

Article  Google Scholar 

Roux, A., Uyhazi, K., Frost, A. & de Camilli, P. GTP-dependent twisting of dynamin implicates constriction and tension in membrane fission. Nature 441, 528–531 (2006).

Article  CAS  Google Scholar 

Tucker, W. C., Weber, T. & Chapman, E. R. Reconstitution of Ca²⁺-regulated membrane fusion by synaptotagmin and SNAREs. Science 304, 435–438 (2004).

Article  CAS  Google Scholar 

Ge, Y. et al. Two forms of Opa1 cooperate to complete fusion of the mitochondrial inner-membrane. eLife 9, e50973 (2020).

Article  CAS  Google Scholar 

Bramkamp, M. Bacterial dynamin-like proteins reveal mechanism for membrane fusion. Nat. Commun. 9, 3993 (2018).

Article  Google Scholar 

van de Cauter, L. et al. Optimized cDICE for efficient reconstitution of biological systems in giant unilamellar vesicles. ACS Synth. Biol. 10, 1690–1702 (2021).

Article  Google Scholar